Objective To analyze the value of multifrequency auditory steady-state response（ASSR） in screening children with different types of hearing impairment. Methods 222 children with congenital hearing impairment diagnosed in our hospital from January 2020 to June 2021 were chosed. 15 common mutation sites of four deafness genes（GJB2

GJB3

SLC26A4 and mitochondrial 12S rRNA） in children’s peripheral blood were detected. Hearing examination included ASSR and click evoked auditory brainstem response（Click-ABR） to judge the degree of hearing impairment. Results In 222 children 105 children were deafness gene mutations

other 117 children had no deafness gene mutations. According to the causes of hearing loss

they were divided into a group of 74 children of conductive hearing impairment

a group of 82 children of sensorineural hearing impairment and a group of 66children of mixed hearing impairment. Compared with non-gene mutation group

the degree of hearing impairment in gene mutation group increased

response thresholds of ASSR and Click-ABR decreased significantly at the frequencies of 0.5

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2 and 4 kHz（P<0.05）; there were no differences of gender and age（P>0.05）. Compared with conductive and sensorineural children

the degree of hearing impairment in the mixed hearing impairment group increased

and the response thresholds of ASSR and Click-ABR decreased significantly at frequencies of 0.5

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2 and 4 kHz（P<0.05）; there were no differences of gender and age（P>0.05）. There were good correlations between ASSR and Click-ABR response threshold at each frequency（P<0.05）. Conclusion The response thresholds of ASSR and Click-ABR are different in children with different types of hearing impairment

including whether there are deafness gene mutations and different causes of hearing impairment. It has a certain auxiliary clinical value for identifying different types of hearing impairment.