浏览全部资源
扫码关注微信
GABAAα1受体亚单位在单侧聋大鼠模型耳蜗核中的表达变化
GABAAα1 Subunit Expression in Cochlear Nucleus of Rats with Single-Side Deafness
- 中国听力语言康复科学杂志 2023年21卷第2期 页码：144-148
- 作者机构：
1.北京中医药大学东直门医院耳鼻喉科 北京 100007
2.中医耳鼻喉头颈研究所 北京 100007
- 作者简介：
原晶晶 硕士 主治医师；研究方向：耳科学与听力学
闫占峰，E-mail： 15210682430@163.com
- 基金信息：北京市自然科学基金资助项目(7214290);北京中医药大学新教师基金启动项目(2022-JYB-XJSJJ-055)
DOI：10.3969/j.issn.1672-4933.2023.02.008
中图分类号：纸质出版日期：2023-03-15，
收稿日期：2022-11-01，
- 稿件说明：
移动端阅览
原晶晶,王鑫,巩政等.GABAAα1受体亚单位在单侧聋大鼠模型耳蜗核中的表达变化[J].中国听力语言康复科学杂志,2023,21(02):144-148.
YUAN Jing-jing,WANG Xin,GONG Zheng,et al.GABAAα1 Subunit Expression in Cochlear Nucleus of Rats with Single-Side Deafness[J].Chinese Scientific Journal of Hearing and Speech Rehabilitation,2023,21(02):144-148.
原晶晶,王鑫,巩政等.GABAAα1受体亚单位在单侧聋大鼠模型耳蜗核中的表达变化[J].中国听力语言康复科学杂志,2023,21(02):144-148. DOI： 10.3969/j.issn.1672-4933.2023.02.008.
YUAN Jing-jing,WANG Xin,GONG Zheng,et al.GABAAα1 Subunit Expression in Cochlear Nucleus of Rats with Single-Side Deafness[J].Chinese Scientific Journal of Hearing and Speech Rehabilitation,2023,21(02):144-148. DOI： 10.3969/j.issn.1672-4933.2023.02.008.
摘要
目的
2
γ-氨基丁酸（γ-aminobutyric acid，GABA）是中枢神经系统重要的抑制性递质，通过观察单侧耳蜗毁损后1、2、4周GABA
A
α1受体亚单位在大鼠耳蜗核的表达变化，为单侧聋听觉中枢重组的分子机制提供参考。
方法
2
新生SD（sprague-dawley）大鼠饲养至出生后12天（P12），随机选择每笼一半数量的大鼠进行单侧耳蜗毁损手术，对照组行伪手术，术后1、2、4周取实验组及对照组大鼠双侧耳蜗核，切片后采用免疫组化方法观察耳蜗核GABA
A
α1受体亚单位的表达变化。
结果
2
实验组术侧及健侧耳蜗核中GABA
A
α1受体亚单位的表达在造模后2周升高，4周降低；实验组术后2周双侧GABA
A
α1受体亚单位表达有显著差异（
P
＜0.01）。实验组术后1、4周双侧耳蜗核GABA
A
α1表达无明显差异（
P
＞0.05）；对照组双侧耳蜗核GABA
A
α1表达在造模后2周升高，4周后下降，对照组大鼠术后1、2、4周双侧耳蜗核GABA
A
α1表达无明显差异（
P
＞0.05）；对照组术侧与实验组术侧相比术后2周有显著差异（
P
＜0.05），术后1、4周有显著差异（
P
＜0.01）。
结论
2
单侧聋大鼠双侧耳蜗核GABA
A
α1受体的不对称改变可能为初级听觉中枢重组所必需。
Abstract
Objective
2
γ- Aminobutyric acid (GABA) is an important inhibitory neurotransmitter of the central nervous system. In this study
we observed the expression of GABA
A
α1 subunits in rat cochlear nucleus 1
2 and 4 weeks after unilateral cochlear lesion.
Methods
2
Newborn sprague-dawley (SD) rats were fed until 12 days after birth (P12). Half of the rats in each cage were randomly selected for unilateral cochlear destruction surgery. The control group was given sham surgery. One week
two weeks and four weeks after surgery
the bilateral cochlear nuclei of the experimental group and the control group were taken. After sectioning
the expression changes of GABA
A
α1 subunits in the cochlear nucleus was observed by immunohistochemistry.
Results
2
In the experimental group
the expression of GABA
A
α1 receptor subunits in the cochlear nucleus of the operative side and the healthy side increased 2 weeks after the model establishment
and decreased 4 weeks after the model establishment; There was a significant difference in the expression of GABA
A
α1 receptor subunits on both sides of the experimental group 2 weeks after operation (
P
<
0.01). There was no significant difference in the expression of GABA
A
α1 in bilateral cochlear nuclei 1 week and 4 weeks after operation in the experimental group (
P
>
0.05); In the control group
the expression of GABA
A
α1 in bilateral cochlear nuclei increased 2 weeks after modeling and decreased 4 weeks after modeling. In the normal control group
there was no significant difference in the expression of GABA in bilateral cochlear nuclei 1
2 and 4 weeks after operation (
P
>
0.05); The difference between the operation side of the control group and the operation side of the experimental group was statistically significant 2 weeks after operation (
P
<
0.05)
and the difference was statistically significant 1 week and 4 weeks after operation (
P
<
0.01).
Conclusion
2
This experiment preliminarily suggests that the asymmetric changes of GABA receptors in bilateral cochlear nuclei of single side deafness rats
which may be necessary for the reorganization of the primary auditory center.
关键词
单侧聋抑制性受体γ-氨基丁酸耳蜗核
Keywords
Single-side deafnessInhibitory receptorγ-Aminobutyric acidCochlear nucleus
references
Popescu MV, Polley DB. Monaural deprivation disrupts development of binaural selectivity in auditory midbrain and cortex[J]. Neuron, 2010, 65 (5): 718-731.
Bajo VM, Nodal FR, Moore DR, et al. The descending corticocollicular pathway mediates learning-induced auditory plasticity[J]. Nature Neuroscience, 2010, 13 (2): 253-260.
Kullmann PH, Ene FA, Kandler K. Glycinergic and GABAergic calcium responses in the developing lateral superior olive[J].Eur J Neurosci, 2002, 15(7):1093-104.
Irvine DRF. Plasticity in the auditory system[J]. Hear Res, 2018, 362(5): 61- 73.
Dai JS, Liu JF, Zhou M,et al. Neurotransmitters and Receptors Changes in Medial Nucleus of the Trapezoid Body (MNTB) of Early-Developmental Rats with Single-Side Deafness[J]. Medical Science Monitor, 2018, 24(1): 397-404.
Wang Y, Xu O, Liu Y, et al. Auditory deprivation modifies the expression of brain-derived neurotrophic factor and tropomyosin receptor kinase B in the rat auditory cortex[J]. Journal of Otology, 2017, 12 (1): 34-40.
Chen Z, Yuan W. Central plasticity and dysfunction elicited by aural deprivation in the critical period[J]. Front Neural Circuits, 2015, 9(6): 1-11 .
刘锦峰, 戴金升, 王宁宇. 单侧听觉剥夺对声源定位通路影响的研究进展[J]. 中华耳鼻咽喉头颈外科杂志, 2017, 52 (2): 136-142.
Diering GH, Huganir RL. The AMPA Receptor Code of Synaptic Plasticity[J]. Neuron, 2018, 100(2):314-329.
Paxinos G,Watson C. The Rat Brain in Stereotaxic Coordinates[M]. London : Academic Pr, 2007.109-121.
Sperk G, Kirchmair E, Bakker J,et al. Immunohistochemical distribution of 10 GABAA receptor subunits in the forebrain of the rhesus monkey Macaca mulatta[J].J Comp Neurol,2020,28(15):2551-2568.
Campos ML, de Cabo C, Wisden W,et al.Expression of GABA(A) receptor subunits in rat brainstem auditory pathways: cochlear nuclei, superior olivary complex and nucleus of the lateral lemniscus[J].Neuroscience,2001,102(3):625-638.
Zhou M, Yuan J, Yan Z, et al. Intrinsic and Miniature Postsynaptic Current Changes in Rat Principal Neurons of the Lateral Superior Olive after Unilateral Auditory Deprivation at an Early Age[J]. Neuroscience,2020, 428(1): 2-12.
Dong S, Mulders WHAM, Rodger Jm, et al.Changes in neuronal activity and gene expression in guinea-pig auditory brainstem after unilateral partial hearing loss[J]. Neuroscience,2009,159(3):1164-1174.
Wang H,Yin G,Rogers K, et al. Monaural conductive hearing loss alters the expression of the GluA3 AMPA and GlyRα1 subunits in bushy and fusiform cells of the cochlear nucleus[J]. Neuroscience, 2011, 199(24): 438-451.
0
浏览量
23
下载量
0
CSCD
- 网络PDF下载
- WORD下载
- Meta-XML下载
- BibTeX下载
- Endnote下载
- RefMan 下载
- NoteExpress下载
- NoteFirst下载
关联资源
相关文章
相关作者
相关机构
- 技术支持由北京北大方正电子有限公司提供 京ICP备2023010756号-1
京公网安备11010502052338 - 本系统建议在Chrome、 IE9+ 以上版本浏览器阅读本站内容，360浏览器请切换至极速模式
- Cookies帮助我们提供服务并提供个性化体验。使用本网站，即表示您同意我们使用Cookies