Drug Distribution and Safety of Dexamethasone Injection after Extracting Perilympha through Round Window

SHI Yan; REN Wei; LI Yu-qing; MA Jing; CAO Da-shan; ZHAO Hui

doi:10.3969/j.issn.1672-4933.2025.03.007

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Drug Distribution and Safety of Dexamethasone Injection after Extracting Perilympha through Round Window

Basic Research | 更新时间：2025-05-12

- Drug Distribution and Safety of Dexamethasone Injection after Extracting Perilympha through Round Window
- Chinese Scientific Journal of Hearing and Speech Rehabilitation Vol. 23, Issue 3, Pages: 251-255(2025)
- 作者机构：
  
  1.华南理工大学医学院广州 510006
  2.解放军总医院第六医学中心北京 100048
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.1672-4933.2025.03.007
  CLC： R764.3
- Received：24 January 2025，
  
  Published：15 May 2025
- 稿件说明：
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石岩,任巍,李雨青等.经圆窗抽取后再注射地塞米松分布及安全性研究[J].中国听力语言康复科学杂志,2025,23(03):251-255.

SHI Yan,REN Wei,LI Yu-qing,et al.Drug Distribution and Safety of Dexamethasone Injection after Extracting Perilympha through Round Window[J].Chinese Scientific Journal of Hearing and Speech Rehabilitation,2025,23(03):251-255.
石岩,任巍,李雨青等.经圆窗抽取后再注射地塞米松分布及安全性研究[J].中国听力语言康复科学杂志,2025,23(03):251-255. DOI： 10.3969/j.issn.1672-4933.2025.03.007.

SHI Yan,REN Wei,LI Yu-qing,et al.Drug Distribution and Safety of Dexamethasone Injection after Extracting Perilympha through Round Window[J].Chinese Scientific Journal of Hearing and Speech Rehabilitation,2025,23(03):251-255. DOI： 10.3969/j.issn.1672-4933.2025.03.007.

摘要

目的

探究豚鼠内耳淋巴液抽取最大安全体积，经圆窗抽取后再注射相同体积药物的安全性和有效性。

方法

取15只正常豚鼠随机化分组，通过内耳外淋巴液梯度抽取后测听确定外淋巴液最大安全抽取体积。后将20只正常豚鼠随机分为空白对照组、全身给药组、经圆窗抽取注射组（用最大安全抽取体积经圆窗抽取外淋巴液后，注射相同体积地塞米松）3组。①有效性实验：通过对比经圆窗抽取注射组与全身给药组处理后2 h内耳药物荧光染色表达，确定其药物分布有效率；②安全性实验：通过对比经圆窗抽取注射组与空白对照组处理后24 h ABR各频段平均阈值确定其操作安全性。

结果

①豚鼠经内耳外淋巴液抽取最大安全剂量为4 μL；②经圆窗抽取注射组地塞米松在内耳分布效率更高；③经圆窗抽取注射组后24 h ABR阈值平均值仅在32 kHz有短期上升，不影响其他频率听阈。

结论

经圆窗抽取后再注射法能实现内耳药物高效分布，短期仅对高频听力有损伤。

Abstract

Objective

Study of the maximum safe volume for lymphatic fluid extraction from the inner ear of guinea pigs

safety and efficacy of the procedure of extracting perilympha and then injecting drugs through a round window membrane (RWM).

Methods

Fifteen normal guinea pigs were taken and randomized into groups

and the maximum safe volume of outer lymphatic fluid to be extracted was determined by gradient extraction of outer lymphatic fluid from the inner ear followed by audiometry.Afterwards

20 normal guinea pigs were randomly divided into 3 groups: a blank control group

a systemic drug administration group

and an extracting perilympha and then injecting drugs via RWM group (group injecting the same volume of dexamethasone after via RWM extracting perilympha with the maximum safe extraction volume).(1)Efficacy experiment: the drug distribution efficiency was determined by comparing the fluorescence staining expression of the drug in the inner ear 2h after treatment.(2)Safety experiment: the safety of the operation was determined by comparing the mean thresholds of each frequency band of ABR 24 h after treatment.

Results

The maximum safe dose for guinea pig lymphatic fluid extraction through the inner ear was 4 μL.Dexamethasone was more efficiently distributed in the group of extracting perilympha and then injecting drugs via RWM.The mean ABR thresholds of the 24h postoperative in the extracting perilympha and then injecting drugs via RWM group had a short-term rise only at 32 kHz and did not affect the hearing thresholds at other frequencies.

Conclusion

The method of extracting perilympha and then injecting drugs via RWM can realize the efficient distribution of inner ear drugs with short-term damage to high-frequency hearing only.

关键词

Keywords

references

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