The Mechanism of Targeted Neural Differentiation of Inner Ear Stem Cells Based on Klf3-As1/Mir-83-5p/Tcf7l2 Axis

JIANG Di; MAO Zhi-qiang; FU Ming

doi:10.3969/j.issn.1672-4933.2025.02.026

您当前的位置：

首页 >

文章列表页 >

The Mechanism of Targeted Neural Differentiation of Inner Ear Stem Cells Based on Klf3-As1/Mir-83-5p/Tcf7l2 Axis

Short Reports | 更新时间：2026-07-11

- The Mechanism of Targeted Neural Differentiation of Inner Ear Stem Cells Based on Klf3-As1/Mir-83-5p/Tcf7l2 Axis
- Chinese Scientific Journal of Hearing and Speech Rehabilitation Vol. 23, Issue 2, Pages: 221-224(2025)
- 作者机构：
  
  南方医科大学第十附属医院（东莞市人民医院）东莞 523000
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.1672-4933.2025.02.026
  CLC： R764.43+1
- Received：30 July 2024，
  
  Published：15 March 2025
- 稿件说明：
移动端阅览
蒋迪,毛志强,傅明.基于KLF3-AS1/miR-83-5p/TCF7L2轴的内耳干细胞定向神经分化作用机制[J].中国听力语言康复科学杂志,2025,23(02):221-224.

JIANG Di,MAO Zhi-qiang,FU Ming.The Mechanism of Targeted Neural Differentiation of Inner Ear Stem Cells Based on Klf3-As1/Mir-83-5p/Tcf7l2 Axis[J].Chinese Scientific Journal of Hearing and Speech Rehabilitation,2025,23(02):221-224.
蒋迪,毛志强,傅明.基于KLF3-AS1/miR-83-5p/TCF7L2轴的内耳干细胞定向神经分化作用机制[J].中国听力语言康复科学杂志,2025,23(02):221-224. DOI： 10.3969/j.issn.1672-4933.2025.02.026.

JIANG Di,MAO Zhi-qiang,FU Ming.The Mechanism of Targeted Neural Differentiation of Inner Ear Stem Cells Based on Klf3-As1/Mir-83-5p/Tcf7l2 Axis[J].Chinese Scientific Journal of Hearing and Speech Rehabilitation,2025,23(02):221-224. DOI： 10.3969/j.issn.1672-4933.2025.02.026.

摘要

目的

探究基于KLF3-AS1/miR-83-5p/TCF7L2轴的内耳干细胞定向神经分化的作用机制。

方法

选取30只SD健康大鼠，分为12只空白组，9只蛋白培养组、9只蛋白培养+LY294002组，对比3组大鼠神经元轴突长度、凋亡率、细胞活性、KLF3-AS1/miR-83-5p/TCF7L2轴相关蛋白表达量。

结果

与空白组、蛋白培养+LY294002组相比，蛋白培养组凋亡率、miR-83-5p蛋白表达量、神经元轴突长度低，KLF3-AS1、细胞活性、TCF7L2蛋白表达量高，差异具有统计学意义（

0.05）。

结论

KLF3-AS1/miR-83-5p/TCF7L2下调可促进内耳干细胞活性，减少凋亡情况，同时在定向神经分化中具有重要作用。

Abstract

Objective

To explore the mechanism of targeted neural differentiation of inner ear stem cells based on KLF3-AS1/miR-83-5p/TCF7L2 axis.

Methods

Thirty healthy SD rats were selected and divided into 12 blank groups

9 protein culture groups and 9 protein culture +LY294002 groups. The neuronal axon length

apoptosis rate

cell activity and axon-related protein expression levels of KLF3-AS1/miR-83-5p/TCF7L2 were compared among the three groups.

Results

Compared with the blank group and the protein culture +LY294002 group

apoptosis rate

miR-83-5p protein expression

neuron axon length were lower in the protein culture group

and KLF3-AS1

cell activity and TCF7L2 protein expression were higher. The difference was statistically significant (

0.05).

Conclusion

Downregulation of KLF3-AS1/miR-83-5p/TCF7L2 can promote the activity of inner ear stem cells

reduce apoptosis

and play an important role in targeted neural differentiation.

关键词

Keywords

references

Connolly K , Gonzalez-Cordero A . Modelling inner ear development and disease using pluripotent stem cells-a pathway to new therapeutic strategies [J ] . Dis Model Mech , 2022 , 15 ( 11 ): 49593 .

Chen F , Yang Y , Chen J , et al . Promotion of In Vitro Hair Cell-like Cell Differentiation from Human Embryonic Stem Cells through the Regulation of Notch Signaling [J ] . Metabolites , 2021 , 11 ( 12 ): 873 .

Chen S , Zhuang Q , Li P , et al . The long non-coding RNAKLF3-AS1/miR-10a-3p/ZBTB20 axis improves the degenerative changes in human nucleus pulposus cells [J ] . Cell Tissue Res , 2023 , 393 ( 1 ): 97 - 109 .

袁佳添 , 谢奇伟 , 王玥 , 等 . 微小RNA通过SIRT1调控小鼠耳蜗老化HEI-OC1细胞凋亡的研究 [J ] . 中国耳鼻咽喉头颈外科 , 2023 , 30 ( 2 ): 84 - 88 .

Guo F , Wang Y . TCF7l2, a nuclear marker that labels premyelinating oligodendrocytes and promotes oligodendroglial lineage progression [J ] . Glia , 2023 , 71 ( 2 ): 143 - 154 .

李丽 , 许翀 , 柴若楠 . LY294002对过敏性鼻炎-哮喘综合征大鼠氧自由基、TRPV1神经元敏感性及TSLP表达的影响 [J ] . 西部医学 , 2023 , 35 ( 9 ): 1276 - 1281 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Research Progress of the Clinical Characteristics and Intervention Models in Presbyphonia

The Development of Behavioral Intervention Approaches for Autism Spectrum Disorder: A Paradigm Shift from Highly Structured Instructional Models to Naturalistic Teaching Strategies

Efficacy of 100 Verb Task Training on the Naming Function of Motor Aphasia after Stroke

Analysis of the Effect of Bilateral Cochlear Implantation and Bimodal Stimulation in Later-implanted Patients with Prelingual Deafness

Analysis of Developmental Delay Monitoring in Children in Xicheng District Beijing

Related Author

No data

Related Institution

No data

⁰