GABA<sub>A</sub>α1 Subunit Expression in Cochlear Nucleus of Rats with Single-Side Deafness

YUAN Jing-jing; WANG Xin; GONG Zheng; ZHANG Ming-sheng; ZHANG Ying; YAN Zhan-feng

doi:10.3969/j.issn.1672-4933.2023.02.008

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GABA_Aα1 Subunit Expression in Cochlear Nucleus of Rats with Single-Side Deafness

Basic Research | 更新时间：2023-05-22

- GABA_Aα1 Subunit Expression in Cochlear Nucleus of Rats with Single-Side Deafness
- Chinese Scientific Journal of Hearing and Speech Rehabilitation Vol. 21, Issue 2, Pages: 144-148(2023)
- 作者机构：
  
  1.北京中医药大学东直门医院耳鼻喉科北京 100007
  2.中医耳鼻喉头颈研究所北京 100007
- 作者简介：
- 基金信息：
- DOI：10.3969/j.issn.1672-4933.2023.02.008
  CLC：
- Published：15 March 2023，
  
  Received：01 November 2022，
- 稿件说明：
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原晶晶,王鑫,巩政等.GABA_Aα1受体亚单位在单侧聋大鼠模型耳蜗核中的表达变化[J].中国听力语言康复科学杂志,2023,21(02):144-148.

YUAN Jing-jing,WANG Xin,GONG Zheng,et al.GABA_Aα1 Subunit Expression in Cochlear Nucleus of Rats with Single-Side Deafness[J].Chinese Scientific Journal of Hearing and Speech Rehabilitation,2023,21(02):144-148.
原晶晶,王鑫,巩政等.GABA_Aα1受体亚单位在单侧聋大鼠模型耳蜗核中的表达变化[J].中国听力语言康复科学杂志,2023,21(02):144-148. DOI： 10.3969/j.issn.1672-4933.2023.02.008.

YUAN Jing-jing,WANG Xin,GONG Zheng,et al.GABA_Aα1 Subunit Expression in Cochlear Nucleus of Rats with Single-Side Deafness[J].Chinese Scientific Journal of Hearing and Speech Rehabilitation,2023,21(02):144-148. DOI： 10.3969/j.issn.1672-4933.2023.02.008.

摘要

目的

γ-氨基丁酸（γ-aminobutyric acid，GABA）是中枢神经系统重要的抑制性递质，通过观察单侧耳蜗毁损后1、2、4周GABA

α1受体亚单位在大鼠耳蜗核的表达变化，为单侧聋听觉中枢重组的分子机制提供参考。

方法

新生SD（sprague-dawley）大鼠饲养至出生后12天（P12），随机选择每笼一半数量的大鼠进行单侧耳蜗毁损手术，对照组行伪手术，术后1、2、4周取实验组及对照组大鼠双侧耳蜗核，切片后采用免疫组化方法观察耳蜗核GABA

α1受体亚单位的表达变化。

结果

实验组术侧及健侧耳蜗核中GABA

α1受体亚单位的表达在造模后2周升高，4周降低；实验组术后2周双侧GABA

α1受体亚单位表达有显著差异（

＜0.01）。实验组术后1、4周双侧耳蜗核GABA

α1表达无明显差异（

＞0.05）；对照组双侧耳蜗核GABA

α1表达在造模后2周升高，4周后下降，对照组大鼠术后1、2、4周双侧耳蜗核GABA

α1表达无明显差异（

＞0.05）；对照组术侧与实验组术侧相比术后2周有显著差异（

＜0.05），术后1、4周有显著差异（

＜0.01）。

结论

单侧聋大鼠双侧耳蜗核GABA

α1受体的不对称改变可能为初级听觉中枢重组所必需。

Abstract

Objective

γ- Aminobutyric acid (GABA) is an important inhibitory neurotransmitter of the central nervous system. In this study

we observed the expression of GABA

α1 subunits in rat cochlear nucleus 1

2 and 4 weeks after unilateral cochlear lesion.

Methods

Newborn sprague-dawley (SD) rats were fed until 12 days after birth (P12). Half of the rats in each cage were randomly selected for unilateral cochlear destruction surgery. The control group was given sham surgery. One week

two weeks and four weeks after surgery

the bilateral cochlear nuclei of the experimental group and the control group were taken. After sectioning

the expression changes of GABA

α1 subunits in the cochlear nucleus was observed by immunohistochemistry.

Results

In the experimental group

the expression of GABA

α1 receptor subunits in the cochlear nucleus of the operative side and the healthy side increased 2 weeks after the model establishment

and decreased 4 weeks after the model establishment; There was a significant difference in the expression of GABA

α1 receptor subunits on both sides of the experimental group 2 weeks after operation (

0.01). There was no significant difference in the expression of GABA

α1 in bilateral cochlear nuclei 1 week and 4 weeks after operation in the experimental group (

0.05); In the control group

the expression of GABA

α1 in bilateral cochlear nuclei increased 2 weeks after modeling and decreased 4 weeks after modeling. In the normal control group

there was no significant difference in the expression of GABA in bilateral cochlear nuclei 1

2 and 4 weeks after operation (

0.05); The difference between the operation side of the control group and the operation side of the experimental group was statistically significant 2 weeks after operation (

0.05)

and the difference was statistically significant 1 week and 4 weeks after operation (

0.01).

Conclusion

This experiment preliminarily suggests that the asymmetric changes of GABA receptors in bilateral cochlear nuclei of single side deafness rats

which may be necessary for the reorganization of the primary auditory center.

关键词

单侧聋抑制性受体γ-氨基丁酸耳蜗核

Keywords

Single-side deafnessInhibitory receptorγ-Aminobutyric acidCochlear nucleus

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Campos ML, de Cabo C, Wisden W,et al.Expression of GABA(A) receptor subunits in rat brainstem auditory pathways: cochlear nuclei, superior olivary complex and nucleus of the lateral lemniscus[J].Neuroscience,2001,102(3):625-638.

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Wang H,Yin G,Rogers K, et al. Monaural conductive hearing loss alters the expression of the GluA3 AMPA and GlyRα1 subunits in bushy and fusiform cells of the cochlear nucleus[J]. Neuroscience, 2011, 199(24): 438-451.

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GABAAα1 Subunit Expression in Cochlear Nucleus of Rats with Single-Side Deafness

DOI：10.3969/j.issn.1672-4933.2023.02.008

摘要

Abstract

关键词

Keywords

references

GABA_Aα1 Subunit Expression in Cochlear Nucleus of Rats with Single-Side Deafness