基于KLF3-AS1/miR-83-5p/TCF7L2轴的内耳干细胞定向神经分化作用机制

蒋迪; 毛志强; 傅明

doi:10.3969/j.issn.1672-4933.2025.02.026

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基于KLF3-AS1/miR-83-5p/TCF7L2轴的内耳干细胞定向神经分化作用机制

短篇报道 | 更新时间：2026-07-11

- 基于KLF3-AS1/miR-83-5p/TCF7L2轴的内耳干细胞定向神经分化作用机制
- The Mechanism of Targeted Neural Differentiation of Inner Ear Stem Cells Based on Klf3-As1/Mir-83-5p/Tcf7l2 Axis
- 中国听力语言康复科学杂志 2025年23卷第2期页码：221-224
- 作者机构：
  
  南方医科大学第十附属医院（东莞市人民医院）东莞 523000
- 作者简介：
  
  蒋迪，博士副主任医师；研究方向：耳神经外科 E-mail：shaouzhixing@163.com
- 基金信息：
  
  广东省自然科学基金“miR-124上调BDNF-TrkB信号通路在内耳干细胞定向分化中的作用及机制研究”(2019A1515110158);广东省自然科学基金“CSF1/CCL22反馈环介导鼻咽癌细胞与TAMs相互作用促鼻咽癌转移的分子机制”(2021B1515140070)
- DOI：10.3969/j.issn.1672-4933.2025.02.026
  中图分类号： R764.43+1
- 收稿：2024-07-30，
  
  纸质出版：2025-03-15
- 稿件说明：
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蒋迪,毛志强,傅明.基于KLF3-AS1/miR-83-5p/TCF7L2轴的内耳干细胞定向神经分化作用机制[J].中国听力语言康复科学杂志,2025,23(02):221-224.

JIANG Di,MAO Zhi-qiang,FU Ming.The Mechanism of Targeted Neural Differentiation of Inner Ear Stem Cells Based on Klf3-As1/Mir-83-5p/Tcf7l2 Axis[J].Chinese Scientific Journal of Hearing and Speech Rehabilitation,2025,23(02):221-224.
蒋迪,毛志强,傅明.基于KLF3-AS1/miR-83-5p/TCF7L2轴的内耳干细胞定向神经分化作用机制[J].中国听力语言康复科学杂志,2025,23(02):221-224. DOI： 10.3969/j.issn.1672-4933.2025.02.026.

JIANG Di,MAO Zhi-qiang,FU Ming.The Mechanism of Targeted Neural Differentiation of Inner Ear Stem Cells Based on Klf3-As1/Mir-83-5p/Tcf7l2 Axis[J].Chinese Scientific Journal of Hearing and Speech Rehabilitation,2025,23(02):221-224. DOI： 10.3969/j.issn.1672-4933.2025.02.026.

摘要

目的

探究基于KLF3-AS1/miR-83-5p/TCF7L2轴的内耳干细胞定向神经分化的作用机制。

方法

选取30只SD健康大鼠，分为12只空白组，9只蛋白培养组、9只蛋白培养+LY294002组，对比3组大鼠神经元轴突长度、凋亡率、细胞活性、KLF3-AS1/miR-83-5p/TCF7L2轴相关蛋白表达量。

结果

与空白组、蛋白培养+LY294002组相比，蛋白培养组凋亡率、miR-83-5p蛋白表达量、神经元轴突长度低，KLF3-AS1、细胞活性、TCF7L2蛋白表达量高，差异具有统计学意义（

0.05）。

结论

KLF3-AS1/miR-83-5p/TCF7L2下调可促进内耳干细胞活性，减少凋亡情况，同时在定向神经分化中具有重要作用。

Abstract

Objective

To explore the mechanism of targeted neural differentiation of inner ear stem cells based on KLF3-AS1/miR-83-5p/TCF7L2 axis.

Methods

Thirty healthy SD rats were selected and divided into 12 blank groups

9 protein culture groups and 9 protein culture +LY294002 groups. The neuronal axon length

apoptosis rate

cell activity and axon-related protein expression levels of KLF3-AS1/miR-83-5p/TCF7L2 were compared among the three groups.

Results

Compared with the blank group and the protein culture +LY294002 group

apoptosis rate

miR-83-5p protein expression

neuron axon length were lower in the protein culture group

and KLF3-AS1

cell activity and TCF7L2 protein expression were higher. The difference was statistically significant (

0.05).

Conclusion

Downregulation of KLF3-AS1/miR-83-5p/TCF7L2 can promote the activity of inner ear stem cells

reduce apoptosis

and play an important role in targeted neural differentiation.

关键词

Keywords

references

Connolly K , Gonzalez-Cordero A . Modelling inner ear development and disease using pluripotent stem cells-a pathway to new therapeutic strategies [J ] . Dis Model Mech , 2022 , 15 ( 11 ): 49593 .

Chen F , Yang Y , Chen J , et al . Promotion of In Vitro Hair Cell-like Cell Differentiation from Human Embryonic Stem Cells through the Regulation of Notch Signaling [J ] . Metabolites , 2021 , 11 ( 12 ): 873 .

Chen S , Zhuang Q , Li P , et al . The long non-coding RNAKLF3-AS1/miR-10a-3p/ZBTB20 axis improves the degenerative changes in human nucleus pulposus cells [J ] . Cell Tissue Res , 2023 , 393 ( 1 ): 97 - 109 .

袁佳添 , 谢奇伟 , 王玥 , 等 . 微小RNA通过SIRT1调控小鼠耳蜗老化HEI-OC1细胞凋亡的研究 [J ] . 中国耳鼻咽喉头颈外科 , 2023 , 30 ( 2 ): 84 - 88 .

Guo F , Wang Y . TCF7l2, a nuclear marker that labels premyelinating oligodendrocytes and promotes oligodendroglial lineage progression [J ] . Glia , 2023 , 71 ( 2 ): 143 - 154 .

李丽 , 许翀 , 柴若楠 . LY294002对过敏性鼻炎-哮喘综合征大鼠氧自由基、TRPV1神经元敏感性及TSLP表达的影响 [J ] . 西部医学 , 2023 , 35 ( 9 ): 1276 - 1281 .

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